File Name: lcms principle and instrumentation .zip
- Mass Spectrometry
- Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS)
- Archives of Organic and Inorganic Chemical Sciences
Liquid Chromatography with tandem mass spectrometry LC-MS-MS is a powerful analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry. A sample solution containing analtyes of interest are pumped through a stationary phase LC column by a mobile phase flowing through at high pressure. Chemical interaction between the components of the sample, the stationary phase and the mobile phase affects different migration rates through the LC column affecting a separation. The wide variety of stationary phase and mobile phase combinations allow for customizing a separation to suit many complex solutions. After elution from the LC column, the effluent is directed to the mass spectrometer.
Liquid chromatography LC is a separation process used to isolate the individual components of a mixture. This process involves mass transfer of a sample through a polar mobile phase and non-polar stationary phase. The device is a column packed with the porous medium made of a granular solid material i. When a sample is injected, it is adsorbed on the stationary phase, and the solvent passes through the column to separate the compounds one by one, based on their relative affinity to the packing materials and the solvent. The component with the most affinity to the stationary phase is the last to separate. This is because high affinity corresponds to more time to travel to the end of the column. High-performance liquid chromatography HPLC , also known as high-pressure liquid chromatography, is an advanced type of LC.
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Liquid Chromatography - Tandem Mass Spectrometry (LC-MS-MS)
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DOI: Abstract PDF. With the Liquid Chromatography LC the separation of components can be done and then the sample eluents from LC are transferred into Mass Spectrometry MS where the detection, identification and determination of masses of components can be done in presence of other components. LC-MS is used in determination, of pharmaceutical drug substances, intermediates and its related compounds for quantitative and qualitative purpose. LC-MS is used most significantly in in-vitro dissolution, bio-equivalence, bioavailability and metabolite studies. Also LC-MS is used in basic research, agrochemical, forensic laboratories and food industries.
With increasingly improved separation of complex samples and detection of unknown material capabilities, liquid chromatography coupled with mass spectrometry LC-MS has been widely used in traditional Chinese medicine TCM research. This article describes the principles of liquid chromatography LC and mass spectrometry MS and their advantages and disadvantages in qualitative and quantitative analysis of TCM. Thousands of years of accumulation of experience on life and disease made by our ancestors finally translated into a modern pharmacy. Chinese herbs, with complex and various ingredients, are usually put into practice by prescription, following the rules of monarch, minister, assistant, and guide. When applied, the amount of single herbs and pharmaceutical formulations can vary, which may lead to changes in the interaction between the drugs and the active ingredient.
Mainly the LC-MS contains liquid chromatography assembly, ion generation unit/ ionization source, The main objective of this review is to overview the principle, instrumentation (solvent degassing system), injector (manual/auto), guard.
Archives of Organic and Inorganic Chemical Sciences
With so many options available, choosing the right sample clean-up method can be bewildering for novice users. The most straightforward of these is to remove proteins and other constituents that may precipitate when injected into the LC mobile phase, to avoid clogging the chromatography column. Depleting or removing these matrix components prevents damage to the column and the build-up of excessive pressure within the LC system.
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Analysis of psychoactive substances, their metabolites and transformation products in environmental samples using LC-MS 3. Comprehensive techniques in LC-MS applications 5. The role of tandem mass spectrometry in clinical chemistry: quantification steroid hormone and Vitamin D
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